| 54 |
Title |
TI |
[EN] SELF-ASSEMBLING PEPTIDE AMPHIPHILES AS RETROVIRAL TRANSDUCTION ENHANCERS
[FR] AMPHIPHILES PEPTIDIQUES À AUTO-ASSEMBLAGE EN TANT QU'ACTIVATEURS DE TRANSDUCTION RÉTROVIRALE |
| 71/73 |
Applicant/owner |
PA |
MAX PLANCK GESELLSCHAFT, DE
|
| 72 |
Inventor |
IN |
HARTENFELS JAN, DE
;
KAYGISIZ KÜBRA, DE
;
MÜNCH JAN, DE
;
RAUCH LENA, DE
;
SYNATSCHKE CHRISTOPHER, DE
;
WEIL TANJA, DE
|
| 22/96 |
Application date |
AD |
May 2, 2024 |
| 21 |
Application number |
AN |
2024062101 |
|
Country of application |
AC |
EP |
|
Publication date |
PUB |
Nov 7, 2024 |
33
31
32 |
Priority data |
PRC
PRN
PRD |
EP
23171437
May 3, 2023
|
33
31
32 |
PRC
PRN
PRD |
EP
23171438
May 3, 2023
|
33
31
32 |
PRC
PRN
PRD |
EP
23206284
Oct 27, 2023
|
| 51 |
IPC main class |
ICM |
C12N 15/87
(2006.01)
|
| 51 |
IPC secondary class |
ICS |
A61K 31/00
(2006.01)
A61K 47/00
(2006.01)
B82Y 5/00
(2011.01)
C07K 7/00
(2006.01)
C07K 7/06
(2006.01)
C07K 7/08
(2006.01)
|
|
IPC additional class |
ICA |
|
|
IPC index class |
ICI |
|
|
Cooperative patent classification |
CPC |
C07K 7/06
C07K 7/08
C12N 15/86
C12N 15/87
C12N 2740/16011
|
|
MCD main class |
MCM |
C12N 15/87
(2006.01)
|
|
MCD secondary class |
MCS |
A61K 31/00
(2006.01)
A61K 47/00
(2006.01)
B82Y 5/00
(2011.01)
C07K 7/00
(2006.01)
C07K 7/06
(2006.01)
C07K 7/08
(2006.01)
|
|
MCD additional class |
MCA |
|
| 57 |
Abstract |
AB |
[EN] The present invention relates to self-assembling peptide amphiphiles consisting of a non-self-assembling peptide moiety having a positive net charge under physiological pH, and a hydrophobic moiety covalently coupled to the peptide moiety, wherein the peptide moiety is composed of a positively charged peptide part and a &bgr;-sheet forming peptide part, wherein the hydrophobic moiety is covalently coupled to the &bgr;-sheet forming peptide part, wherein the peptide amphiphiles self-assemble into fibrils and form µm-sized aggregates in aqueous medium, and wherein the fibrils are efficiently degradable in cells. The peptide amphiphiles of the present invention are useful as viral transduction enhancers for retroviral gene delivery into cells. Thus, the present invention further relates to the use of peptide amphiphiles as retroviral transduction enhancers in retroviral gene delivery into cells, and to methods for retroviral gene delivery into cells in presence of peptide amphiphiles.
[FR] La présente invention concerne des amphiphiles peptidiques à auto-assemblage constitués d'une fraction peptidique non auto-assemblable présentant une charge nette positive à pH physiologique, et d'une fraction hydrophobe couplée de manière covalente à la fraction peptidique, la fraction peptidique étant composée d'une partie peptidique chargée positivement et d'une partie peptidique constituant un feuillet &bgr;, le groupement hydrophobe est couplé de manière covalente à la partie peptidique formant un feuillet &bgr;, les amphiphiles peptidiques s'auto-assemblant en fibrilles et constituant des agrégats de taille µm en milieu aqueux, et les fibrilles étant efficacement dégradables dans les cellules. Les amphiphiles peptidiques de la présente invention sont utiles en tant qu'activateurs de transduction virale pour l'administration de gènes rétroviraux dans des cellules. Ainsi, la présente invention concerne en outre l'utilisation d'amphiphiles peptidiques en tant qu'activateurs de transduction rétrovirale dans l'administration de gènes rétroviraux dans des cellules, et des procédés d'administration de gènes rétroviraux dans des cellules en présence d'amphiphiles peptidiques. |
| 56 |
Cited documents identified in the search |
CT |
WO002003054146A2
|
| 56 |
Cited documents indicated by the applicant |
CT |
|
| 56 |
Cited non-patent literature identified in the search |
CTNP |
KAYGISIZ K ET AL: "Peptide Amphiphiles as Biodegradable Adjuvants for Efficient Retroviral Gene Delivery", ADVANCED HEALTHCARE MATERIALS, WILEY - V C H VERLAG GMBH & CO. KGAA, DE, vol. 13, no. 4, 23 November 2023 (2023-11-23), pages n/a, XP072582214, ISSN: 2192-2640, DOI: 10.1002/ADHM.202301364 7;
KAYGISIZ KÜBRA ET AL: "Supporting Information", 23 November 2023 (2023-11-23), pages 1 - 49, XP093191156, Retrieved from the Internet 7;
KOLBERG-EDELBROCK J ET AL: "Biomimetic Extracellular Scaffolds by Microfluidic Superstructuring of Nanofibers", vol. 9, no. 3, 21 February 2023 (2023-02-21), pages 1251 - 1260, XP093092438, ISSN: 2373-9878, Retrieved from the Internet DOI: 10.1021/acsbiomaterials.2c01098 7;
SIESTE S ET AL: "Supramolecular Peptide Nanofibrils with Optimized Sequences and Molecular Structures for Efficient Retroviral Transduction", vol. 31, no. 17, 22 February 2021 (2021-02-22), DE, pages 1 - 12, XP093092532, ISSN: 1616-301X, Retrieved from the Internet DOI: 10.1002/adfm.202009382 7
|
| 56 |
Cited non-patent literature indicated by the applicant |
CTNP |
|
|
Citing documents |
|
Determine documents
|
|
Sequence listings |
|
|
|
Search file IPC |
ICP |
A61K 31/00
B82Y 5/00
C12N 15/86
C12N 15/87
|
