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Inventor |
IN |
BOECKER SIMON, DE
;
MEYER VERA, DE
;
MÜHLENWEG AGNES, DE
;
RICHTER LENNART, DE
;
STORM DIRK, DE
;
SÜSSMUTH RODERICH, DE
;
WANKA FRANZISKA, DE
;
ZOBEL SOPHIA, DE
|
| 57 |
Abstract |
AB |
[EN] The present invention relates to a method for obtaining at least one microbial secondary metabolite or a derivative thereof, the method comprising the step of heterologous expression of at least one synthetase of said secondary metabolite in at least one filamentous fungus. Furthermore, the present invention relates to a expression cassette, a plasmid vector containing said expression cassette, an expression host, novel cyclodepsipeptides and a chimeric cyclodepsipeptide synthetase.
[FR] La présente invention concerne un procédé d'obtention d'au moins un métabolite secondaire microbienne ou d'un dérivé de celui-ci, le procédé comprenant l'étape d'expression hétérologue d'au moins une synthétase dudit métabolite secondaire dans au moins un champignon filamenteux. En outre, la présente invention concerne une cassette d'expression, un vecteur plasmidique contenant ladite cassette d'expression, un hôte d'expression, de nouveaux cyclodepsipeptides et une synthétase de cyclodepsipeptide chimère. |
| 56 |
Cited non-patent literature identified in the search |
CTNP |
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MADRY, N. ET AL.: "Enniatin production by Fusarium oxysporum in chemically defined media", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 17, no. 2, March 1983 (1983-03-01), pages 75 - 79, XP035171867 7;
MEYER, V. ET AL.: "Fungal Gene Expression on Demand: An Inducible, Tunable, and Metabolism-Independent Expression System for Aspergillus niger", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 77, no. 9, May 2011 (2011-05-01), pages 2975 - 2983, XP002740895 7;
RICHTER, L. ET AL.: "Engineering of Aspergillus niger for the production of secondary metabolites", FUNGAL BIOLOGY AND BIOTECHNOLOGY, vol. 1, 14 October 2014 (2014-10-14), pages 4, XP021200577 7;
SÜSSMUTH, R. ET AL.: "Fungal cyclooligomer depsipeptides: From classical biochemistry to combinatorial biosynthesis", NATURAL PRODUCT REPORTS, vol. 28, no. 1, 20 October 2010 (2010-10-20), pages 99 - 124, XP002740896 7;
YU, D. ET AL.: "Functional dissection and module swapping of fungal cyclooligomer depsipeptide synthetases", CHEMICAL COMMUNICATIONS, 14 July 2013 (2013-07-14), pages 6176 - 6178, XP002740894 7
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