Bibliografische Daten

Dokument WO002014114810A1 (Seiten: 82)

Bibliografische Daten Dokument WO002014114810A1 (Seiten: 82)
INID Kriterium Feld Inhalt
54 Titel TI [EN] NOVEL GLUCOSE OXIDASES DERIVED FROM ASPERGILLUS NIGER
[FR] NOUVELLES GLUCOSE OXYDASES DÉRIVÉES D'ASPERGILLUS NIGER
71/73 Anmelder/Inhaber PA HOFFMANN LA ROCHE, CH ; ROCHE DIAGNOSTICS GMBH, DE
72 Erfinder IN BOCOLA MARCO, DE ; DUEFEL HARTMUT, DE ; GUTIERREZ ERIK UWE, DE ; HEINDL DIETER, DE ; MEIER THOMAS, DE ; MUNDHADA HEMANSHU, DK ; SCHWANEBERG ULRICH, BE ; TACKE MICHAEL, DE
22/96 Anmeldedatum AD 28.01.2014
21 Anmeldenummer AN 2014051602
Anmeldeland AC EP
Veröffentlichungsdatum PUB 31.07.2014
33
31
32
Priorität PRC
PRN
PRD
EP
13152935
20130128
51 IPC-Hauptklasse ICM C12N 9/54 (2006.01)
51 IPC-Nebenklasse ICS
IPC-Zusatzklasse ICA
IPC-Indexklasse ICI
Gemeinsame Patentklassifikation CPC C12N 9/0006
C12N 9/54
C12Q 1/54
C12Y 101/03004
G01N 2333/904
MCD-Hauptklasse MCM C12N 9/54 (2006.01)
MCD-Nebenklasse MCS
MCD-Zusatzklasse MCA
57 Zusammenfassung AB [EN] Disclosed herein are novel glucose oxidase variants having besides the two substitutions T30V and I94V according to SEQ ID NO: 1, additionally at least one further amino acid substitution in the polypeptide sequence in any of the positions S53; A137; A173; A332; F414; and V560. The thus optimized glucose oxidase variants exhibit specificity for glucose and significantly reduced oxygen-consumption rates. In another aspect, the present invention provides for glucose oxidase variants having significantly reduced oxygen-consumption rates and/or increased enzyme activity for electron mediators other than oxygen. The invention also provides for an assay device bearing at least one of the herein disclosed GOx variants for improved blood glucose measurements.
[FR] L'invention concerne de nouveaux variants de glucose oxydase comportant, outre les deux substitutions T30V et I94V conformément à SEQ ID NO: 1, au moins une autre substitution d'acide aminé dans la séquence polypeptidique dans l'une quelconque des positions S53, A137, A173, A332, F414 et V560. Les variants de glucose oxydase ainsi optimisés présentent une spécificité pour le glucose et des taux de consommation d'oxygène significativement réduits. Dans un autre aspect, la présente invention concerne des variants de glucose oxydase ayant des taux de consommation d'oxygène significativement réduits et/ou une activité enzymatique accrue pour les médiateurs d'électrons autres que l'oxygène. L'invention concerne également un dispositif de test portant au moins un des variants de GOx décrits ici pour améliorer les mesures de glucose sanguin.
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