| 54 |
Titel |
TI |
[EN] STEREOSELECTIVE HYDROXYLATION OF BENZENES
[FR] HYDROXYLATION STÉRÉOSÉLECTIVE DES BENZÈNES |
| 71/73 |
Anmelder/Inhaber |
PA |
DSM IP ASSETS BV, NL
;
RWTH AACHEN, DE
|
| 72 |
Erfinder |
IN |
BONRATH WERNER, CH
;
DENNIG ALEXANDER, DE
;
MARIENHAGEN JAN, DE
;
RUFF ANNA JOELLE, BE
;
SCHUERMANN MARTIN, NL
;
SCHWANENBERG ULRICH, BE
|
| 22/96 |
Anmeldedatum |
AD |
24.04.2013 |
| 21 |
Anmeldenummer |
AN |
2013058525 |
|
Anmeldeland |
AC |
EP |
|
Veröffentlichungsdatum |
PUB |
31.10.2013 |
33
31
32 |
Priorität |
PRC
PRN
PRD |
EP
12165386
20120424
|
| 51 |
IPC-Hauptklasse |
ICM |
C12P 33/06
(2006.01)
|
| 51 |
IPC-Nebenklasse |
ICS |
C12N 9/00
(2006.01)
C12N 9/02
(2006.01)
|
|
IPC-Zusatzklasse |
ICA |
|
|
IPC-Indexklasse |
ICI |
|
|
Gemeinsame Patentklassifikation |
CPC |
C12N 9/0071
C12P 17/06
C12P 7/22
C12Y 114/14001
|
|
MCD-Hauptklasse |
MCM |
C12P 33/06
(2006.01)
|
|
MCD-Nebenklasse |
MCS |
C12N 9/00
(2006.01)
C12N 9/02
(2006.01)
|
|
MCD-Zusatzklasse |
MCA |
|
| 57 |
Zusammenfassung |
AB |
[EN] The present invention relates to novel cytochrome P450 monooxygenase with modified (higher) substrate selectivity and/or (re)activity towards aromatic hydroxylation of benzenes. In particular, the invention provides nucleic acid sequences coding for the novel enzymes, expression constructs and vectors comprising these sequences, transformed microorganisms expressing these novel enzymes, and processes for the stereoselective hydroxylation of substituted benzenes useful as building blocks in vitamin E synthesis.
[FR] Cette invention concerne une nouvelle cytochrome P450 monooxygénase ayant une sélectivité de substrat et/ou une (ré)activité vis-à-vis de l'hydroxylation des benzènes modifiées (supérieures). En particulier, l'invention concerne des séquences d'acides nucléiques codant pour les nouvelles enzymes, des produits et recombinaison et des vecteurs d'expression les contenant, des micro-organismes transformés exprimant ces nouvelles enzymes, et des procédés d'hydroxylation stéréosélective de benzènes substitués utiles à titre d'éléments structuraux dans la synthèse de la vitamine E. |
| 56 |
Entgegengehaltene Patentdokumente/Zitate,
in Recherche ermittelt |
CT |
WO002009047498A2
WO002010064764A1
|
| 56 |
Entgegengehaltene Patentdokumente/Zitate,
vom Anmelder genannt |
CT |
|
| 56 |
Entgegengehaltene Nichtpatentliteratur/Zitate,
in Recherche ermittelt |
CTNP |
CARMICHAEL A B ET AL: "Protein engineering of Bacillus megaterium CYP102.The oxidation of polycyclic aromatic hydrocarbons", EUROPEAN JOURNAL OF BIOCHEMISTRY, BLACKWELL PUBLISHING, BERLIN, DE, vol. 268, no. 10, 1 May 2001 (2001-05-01), pages 3117 - 3125, XP002449232, ISSN: 0014-2956, DOI: 10.1046/J.1432-1327.2001.02212.X 7;
CHRISTOPHER J. C. WHITEHOUSE ET AL: "Dearomatisation of o-Xylene by P450BM3 (CYP102A1)", CHEMISTRY - A EUROPEAN JOURNAL, vol. 17, no. 24, 6 June 2011 (2011-06-06), pages 6862 - 6868, XP055041233, ISSN: 0947-6539, DOI: 10.1002/chem.201002465 7;
MUNRO A W ET AL: "P450 BM3: the very model of a modern flavocytochrome", TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER, HAYWARDS, GB, vol. 27, no. 5, 1 May 2002 (2002-05-01), pages 250 - 257, XP004366127, ISSN: 0968-0004, DOI: 10.1016/S0968-0004(02)02086-8 7;
OLIVER C F ET AL: "Engineering the substrate specificity of Bacillus megaterium cytochrome P-450 BM3: Hydroxylation of alkyl trimethylammonium compounds", BIOCHEMICAL JOURNAL, THE BIOCHEMICAL SOCIETY, LONDON, GB, vol. 327, no. 2, 1 January 1997 (1997-01-01), pages 537 - 544, XP002156031, ISSN: 0264-6021 7;
S. A. BARDOWELL ET AL: "Common Variants of Cytochrome P450 4F2 Exhibit Altered Vitamin E- -Hydroxylase Specific Activity", THE JOURNAL OF NUTRITION, vol. 140, no. 11, 1 November 2010 (2010-11-01), pages 1901 - 1906, XP055041219, ISSN: 0022-3166, DOI: 10.3945/jn.110.128579 7
|
| 56 |
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vom Anmelder genannt |
CTNP |
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