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Dokument US000007501276B2 (Seiten: 12)

Bibliografische Daten Dokument US000007501276B2 (Seiten: 12)
INID Kriterium Feld Inhalt
54 Titel TI [EN] Apparatus for intracellular manipulation of a biological cell
71/73 Anmelder/Inhaber PA MICRONAS GMBH, DE
72 Erfinder IN BAUMANN WERNER, DE ; BRISCHWEIN MARTIN, DE ; EHRET RALF, DE ; FREUND INGO, DE ; GAHLE HANS-JUERGEN, DE ; IGEL GUENTER, DE ; LEHMANN MIRKO, DE ; SIEBEN ULRICH, DE
22/96 Anmeldedatum AD 23.07.2002
21 Anmeldenummer AN 20241902
Anmeldeland AC US
Veröffentlichungsdatum PUB 10.03.2009
33
31
32
Priorität PRC
PRN
PRD
DE
19823655
27.05.1998
33
31
32
PRC
PRN
PRD
DE
19841337
10.09.1998
33
31
32
PRC
PRN
PRD
DE
29811066
23.06.1998
33
31
32
PRC
PRN
PRD
US
32105199
27.05.1999
51 IPC-Hauptklasse ICM C12M 1/42 (2006.01)
51 IPC-Nebenklasse ICS C12N 15/87 (2006.01)
IPC-Zusatzklasse ICA
IPC-Indexklasse ICI
Gemeinsame Patentklassifikation CPC C12M 35/02
C12M 35/04
C12M 35/08
C12N 13/00
C12N 15/89
MCD-Hauptklasse MCM C12M 1/42 (2006.01)
C12N 15/09 (2006.01)
MCD-Nebenklasse MCS C12M 1/00 (2006.01)
C12M 3/00 (2006.01)
C12N 13/00 (2006.01)
C12N 15/87 (2006.01)
C12N 15/89 (2006.01)
C12N 5/02 (2006.01)
MCD-Zusatzklasse MCA
57 Zusammenfassung AB [EN] A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of the cell (3) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (5), thus seals off the cell fluid situated in the interior of the cell (3) from the culture medium (2) and insulates the cell fluid against the culture medium (2). The interior of the cell (3) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier (4) with a support area (5) for adhering the cell and a poration tool (6) for creating the opening in the cell membrane. The poration tool (6) may be any of various chemical, mechanical and/or electrical devices.
56 Entgegengehaltene Patentdokumente/Zitate,
in Recherche ermittelt
CT JP000H01223348A
JP000S63222680A
JP000S63269977A
US000004619899A
US000004784737A
US000005795755A
US000006156576A
US000006368851B1
56 Entgegengehaltene Patentdokumente/Zitate,
vom Anmelder genannt
CT DE000004004198A1
DE000004031138A1
DE000004400955A1
EP000000292899A2
EP000000539660A2
US000005232856A
US000005262128A
US000005457041A
WO001995023211A1
WO001997017426A1
56 Entgegengehaltene Nichtpatentliteratur/Zitate,
in Recherche ermittelt
CTNP Hengstenberg. "A piezoelectric device to aid penetration of small nerve fibers with microelectrodes". Journal of Neuroscience Methods. vol. 4, No. 3 (Oct. 1981), Abstract Only. 0;
Horn et al. "Muscarinic Activation of Ionic Currents Measured by a New Whole-Cell Recording Method". J. Gen. Physiol. vol. 92 (Aug. 1988), pp. 145-159. 0
56 Entgegengehaltene Nichtpatentliteratur/Zitate,
vom Anmelder genannt
CTNP Alberts, B. et al. Molecular Biology of the Cell, 3rd edition, Garland Publishing, New York, N.Y., pp. 181-191, 1051-1059, and 1131-1132 (1994). 1;
Bao, S. et al., "Ultrasound Induced Gene Transfection and its Implications in Cancer Gene Therapy," Conference Proceedings, vol. 43 (suppl2): S82; (1997). 1;
Harvey, A.N. et al., "Electroporation and streptolysis O-a comparison of poration techniques," Elsevier, Mutation Research, DNA Repair 315 pp. 17-23 (1994). 1;
Kaupp, U.B., "Nobel Prize for Medicine-Measurement of Flow in Discrete Channels," translation of article from Spektrum der Wissenschaft pp. 22-24 (Dec. 1991). 1;
Kober, L.O. et al. "Effect of the pulse length of ultrasound on cell membrane damage in vitro," Journal of the Acoustical Society of America 86(1) pp. 6-7 (Jul. 1989). 1;
Lundqvist et al., "Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes." Proc. Natl. Acad. Sci. USA, 95:10356-10360 (Sep. 1998). 1;
Lundqvist et al., "Analyte-Transfer into Single Cells and Cytoplasmic Analysis Using Miniaturized Electroporation and CE-LIF Detection," PITTCON '98, Poster Paper 1604P (Mar. 1998). 1;
Prausnitz, M.R. et al. "Size, Lifetime and Permeability of Ultrasound-Mediated Cell Membrane Disruptions," 1999 Bioengineering Conference, Paper a0136629 (1999). 1;
Schütze, K. et al. "Cut out or poke in-the key to the world of single genes: laser micro-manipulation as a valuable tool on the look-out for the origin of disease," Genetic Analysis: Biomolecular Engineering, 14 pp. 1-8, (1997). 1;
Teruel et al., "Electroporation-Induced Formation of Individual Calcium Entry Sites in the Cell Body and Processes of Adherent Cells," Biophsical Journal, 73:1785-1796 (Oct. 1997). 1;
Worle, K. et al., "The combined effects of high-energy shock waves and cytostatic drugs or cytokines on human bladder cancer cells," British Journal of Cancer 69(1):58-65, (abstract only), (Jan. 1994). 1
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Prüfstoff-IPC ICP C12M 1/42
C12N 15/87