Bibliographic data

Document US000007226768B2 (Pages: 84)

Bibliographic data Document US000007226768B2 (Pages: 84)
INID Criterion Field Contents
54 Title TI [EN] Cytochrome P450 oxygenases
71/73 Applicant/owner PA CALIFORNIA INST OF TECHN, US
72 Inventor IN ARNOLD FRANCES H, US ; FARINAS EDGARDO T, US ; GLIEDER ANTON, AT ; SCHWANEBERG ULRICH, DE
22/96 Application date AD Jul 22, 2002
21 Application number AN 20121302
Country of application AC US
Publication date PUB Jun 5, 2007
33
31
32		 Priority data PRC
PRN
PRD		 US
30676601
Jul 20, 2001
33
31
32		 PRC
PRN
PRD		 US
30842901
Jul 27, 2001
51 IPC main class ICM C12N 9/02 (2006.01)
51 IPC secondary class ICS C07H 21/04 (2006.01)
C12N 1/20 (2006.01)
C12N 15/00 (2006.01)
C12P 21/04 (2006.01)
C12Q 1/26 (2006.01)
C12Q 1/34 (2006.01)
IPC additional class ICA C12Q 1/00 (2006.01)
IPC index class ICI
Cooperative patent classification CPC C12N 9/0071
C12P 17/02
C12P 7/02
C12P 7/04
C12P 7/16
Y02E 50/10
Y02P 20/52
MCD main class MCM C12N 9/02 (2006.01)
MCD secondary class MCS C07H 21/04 (2006.01)
C12N 1/20 (2006.01)
C12N 15/00 (2006.01)
C12P 17/02 (2006.01)
C12P 21/04 (2006.01)
C12P 7/02 (2006.01)
C12P 7/04 (2006.01)
C12P 7/16 (2006.01)
C12Q 1/26 (2006.01)
C12Q 1/34 (2006.01)
MCD additional class MCA C12Q 1/00 (2006.01)
57 Abstract AB [EN] Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
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Citing documents Determine documents
Sequence listings
Search file IPC ICP C12N 1/20
C12N 15/00
C12N 9/02
C12P 21/04
C12P 21/06
C12Q 1/00
C12Q 1/26
C12Q 1/34