| 54 |
Titel |
TI |
[EN] Method for intracellular manipulation of a biological cell |
| 71/73 |
Anmelder/Inhaber |
PA |
MICRONAS GMBH, DE
|
| 72 |
Erfinder |
IN |
BAUMANN WERNER, DE
;
BRISCHWEIN MARTIN, DE
;
EHRET RALF, DE
;
FREUND INGO, DE
;
GAHLE HANS-JUERGEN, DE
;
IGEL GUENTER, DE
;
LEHMANN MIRKO, DE
;
SIEBEN ULRICH, DE
;
WOLF BERNHARD, DE
|
| 22/96 |
Anmeldedatum |
AD |
27.05.1999 |
| 21 |
Anmeldenummer |
AN |
32105199 |
|
Anmeldeland |
AC |
US |
|
Veröffentlichungsdatum |
PUB |
05.11.2002 |
33
31
32 |
Priorität |
PRC
PRN
PRD |
DE
19823655
27.05.1998
|
33
31
32 |
PRC
PRN
PRD |
DE
19841337
10.09.1998
|
33
31
32 |
PRC
PRN
PRD |
DE
29811066
23.06.1998
|
| 51 |
IPC-Hauptklasse |
ICM |
C12N 13/00
|
| 51 |
IPC-Nebenklasse |
ICS |
|
|
IPC-Zusatzklasse |
ICA |
|
|
IPC-Indexklasse |
ICI |
|
|
Gemeinsame Patentklassifikation |
CPC |
C12M 35/02
C12M 35/04
C12M 35/08
C12N 13/00
C12N 15/89
|
|
MCD-Hauptklasse |
MCM |
C12N 15/09
(2006.01)
|
|
MCD-Nebenklasse |
MCS |
C12M 1/00
(2006.01)
C12M 1/42
(2006.01)
C12M 3/00
(2006.01)
C12N 13/00
(2006.01)
C12N 15/89
(2006.01)
C12N 5/02
(2006.01)
|
|
MCD-Zusatzklasse |
MCA |
|
| 57 |
Zusammenfassung |
AB |
[EN] A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of the cell (3) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (5), thus seals off the cell fluid situated in the interior of the cell (3) from the culture medium (2) and insulates the cell fluid against the culture medium (2). The interior of the cell (3) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier (4) with a support area (5) for adhering the cell and a poration tool (6) for creating the opening in the cell membrane. The poration tool (6) may be any of various chemical, mechanical and/or electrical devices. |
| 56 |
Entgegengehaltene Patentdokumente/Zitate,
in Recherche ermittelt |
CT |
US000005232856A
WO001995023211A1
|
| 56 |
Entgegengehaltene Patentdokumente/Zitate,
vom Anmelder genannt |
CT |
DE000004004198A1
DE000004031138A1
DE000004400955A1
EP000000292899A2
EP000000539660A2
US000005262128A
US000005457041A
WO001997017426A1
|
| 56 |
Entgegengehaltene Nichtpatentliteratur/Zitate,
in Recherche ermittelt |
CTNP |
Lundqvist et al., (1998) PITTCON '98, Poster Paper 1604P. 0;
Lundqvist et al., (1998) PNAS vol. 95, pp. 10356-10360.* 0;
Teruel and Meyer, (1997) Biophys. J. vol. 73, pp. 1785-1786.* 0
|
| 56 |
Entgegengehaltene Nichtpatentliteratur/Zitate,
vom Anmelder genannt |
CTNP |
|
|
Zitierende Dokumente |
|
Dokumente ermitteln
|
|
Sequenzprotokoll |
|
|
|
Prüfstoff-IPC |
ICP |
C12M 1/26 ZEL
C12M 1/34 PC
C12M 1/42
C12N 13/00
C12N 15/89
|
