| 54 |
Title |
TI |
[EN] NOVEL CYTOCHROME P-450 MONOOXYGENASES AND THEIR USING FOR OXIDATION OF ORGANIC COMPOUNDS
[XX] НОВЫЕ ЦИТОХРОМ Р450-МОНООКСИГЕНАЗЫ И ИХ ПРИМЕНЕНИЕ ДЛЯ ОКИСЛЕНИЯ ОРГАНИЧЕСКИХ СОЕДИНЕНИЙ |
| 71/73 |
Applicant/owner |
PA |
BASF AG, DE
|
| 72 |
Inventor |
IN |
APPEL DANIEHL, DE
;
FISHER MARKUS, DE
;
KHAUEHR BERNKHARD, DE
;
LI KUING-SHAN, JP
;
LJUTTS-VAL SABINE, DE
;
PLJAJSS JUERGEN, DE
;
SHMID ROL F, DE
;
SHMITT JUTTA, DE
;
SHVANEBERG UL RIKH, DE
|
| 22/96 |
Application date |
AD |
Jul 27, 2000 |
| 21 |
Application number |
AN |
2002105499 |
|
Country of application |
AC |
RU |
|
Publication date |
PUB |
Oct 10, 2006 |
33
31
32 |
Priority data |
PRC
PRN
PRD |
DE
19935115
Jul 27, 1999
|
33
31
32 |
PRC
PRN
PRD |
DE
19955605
Nov 18, 1999
|
| 51 |
IPC main class |
ICM |
C12N 15/53
(2006.01)
|
| 51 |
IPC secondary class |
ICS |
C12N 9/02
(2006.01)
C12P 17/10
(2006.01)
C12P 17/16
(2006.01)
C12P 7/04
(2006.01)
C12P 7/22
(2006.01)
|
|
IPC additional class |
ICA |
|
|
IPC index class |
ICI |
|
|
Cooperative patent classification |
CPC |
|
|
MCD main class |
MCM |
C12N 15/53
(2006.01)
|
|
MCD secondary class |
MCS |
C12N 9/02
(2006.01)
C12P 17/10
(2006.01)
C12P 17/16
(2006.01)
C12P 7/04
(2006.01)
C12P 7/22
(2006.01)
|
|
MCD additional class |
MCA |
|
| 57 |
Abstract |
AB |
[EN] FIELD: biotechnology, biochemistry, enzymes, microbiology. ^ SUBSTANCE: invention proposes a method for microbiological oxidation of N-, O- or S-heterocyclic mono- or multinuclear aromatic compounds. The method involves culturing the recombinant microorganism that expresses cytochrome P-450-dependent monooxygenase BM-3 from Bacillus megaterium with amino acid sequence represented in SEQ ID NO:2 that comprises at least one functional mutation in region 86-88 and, if necessary, at least one functional mutation in one of regions 73-82, 172-224, 39-43, 48-52, 67-170, 300-335 and 352-356. The prepared oxidation product is isolated from medium, Invention provides carrying out oxidation of organic compounds with enhanced degree of effectiveness. ^ EFFECT: enhanced effectiveness of enzymes. ^ 17 cl, 1 tbl, 7 ex |
| 56 |
Cited documents identified in the search |
CT |
|
| 56 |
Cited documents indicated by the applicant |
CT |
|
| 56 |
Cited non-patent literature identified in the search |
CTNP |
|
| 56 |
Cited non-patent literature indicated by the applicant |
CTNP |
|
|
Citing documents |
|
Determine documents
|
|
Sequence listings |
|
|
|
Search file IPC |
ICP |
|
