54 |
Title |
TI |
[EN] METHOD FOR MEASURING THE QUANTITY OF STATE |
71/73 |
Applicant/owner |
PA |
MICRONAS INTERMETALL GMBH
|
72 |
Inventor |
IN |
BAUMANN WERNER
;
BRISCHWEIN MARTIN DR
;
EHRET RALF
;
FREUND INGO
;
GAHLE JUERGEN
;
IGEL GUENTER
;
LEHMANN MIRKO
;
SIEBEN ULRICH
;
WOLF BERNHARD
|
22/96 |
Application date |
AD |
May 25, 1999 |
21 |
Application number |
AN |
14528499 |
|
Country of application |
AC |
JP |
|
Publication date |
PUB |
Dec 21, 1999 |
33 31 32 |
Priority data |
PRC PRN PRD |
DE
19823655
May 27, 1998
|
33 31 32 |
PRC PRN PRD |
DE
29811066
Jun 23, 1998
|
51 |
IPC main class |
ICM |
C12Q 1/02
|
51 |
IPC secondary class |
ICS |
C12M 1/00
|
|
IPC additional class |
ICA |
|
|
IPC index class |
ICI |
|
|
Cooperative patent classification |
CPC |
C12M 35/02
C12M 35/04
C12M 35/08
C12N 13/00
C12N 15/89
|
|
MCD main class |
MCM |
C12M 1/00
(2006.01)
|
|
MCD secondary class |
MCS |
C12M 3/00
(2006.01)
C12N 13/00
(2006.01)
C12N 15/89
(2006.01)
C12Q 1/02
(2006.01)
|
|
MCD additional class |
MCA |
|
57 |
Abstract |
AB |
[EN] PROBLEM TO BE SOLVED: To simply measure the quantity of the state of biological cells in no need of the expensive positioning by boring a fine hole on the cell membrane within the cell-supporting range at an interval from its edge and measuring the quantity of the state of biological cells adhering and accumulating through the fine hole (pore). SOLUTION: In the measurement of at least one of quantities of the states of biological cells 3 adhering in the supporting range 5 in the culture medium where at least one minute hole is bored on the cell membrane of the cell 3 in order to measure the quantity of a state, the cell 3 is placed on the base plate 21 of the microscope stage 4 and a fine hole is bored in the supporting range 5 of the cell 3 at an interval from the edge by the electric poring using a test probe comprising the measuring electrode 6 and the electric fine hole- boring electrode 7. Then, the cell potential is measured using at least one reference electrode that can contact through the fine hole with the measuring electrode 6 and the culture medium. Thus, this measuring method can avoid the manual, expensive positioning operation of the cell to be inspected with a fine hollow needle. |
56 |
Cited documents identified in the search |
CT |
|
56 |
Cited documents indicated by the applicant |
CT |
|
56 |
Cited non-patent literature identified in the search |
CTNP |
|
56 |
Cited non-patent literature indicated by the applicant |
CTNP |
|
|
Citing documents |
|
Determine documents
|
|
Sequence listings |
|
|
|
Search file IPC |
ICP |
C12M 1/26 ZEL
C12M 1/34 PC
C12M 1/34
C12M 1/42
C12N 13/00
C12N 15/89
C12Q 1/02
|