| 54 |
Title |
TI |
[EN] METHOD FOR INTRACELLULARLY OPERATING BIOLOGICAL CELL AND DEVICE THEREFOR |
| 71/73 |
Applicant/owner |
PA |
MICRONAS INTERMETALL GMBH
|
| 72 |
Inventor |
IN |
BAUMANN WERNER
;
BRISCHWEIN MARTIN DR
;
EHRET RALF
;
FREUND INGO
;
GAHLE HANS-J
;
IGEL GUENTER
;
LEHMANN MIRKO
;
SIEBEN ULRICH
;
WOLF BERNHARD
|
| 22/96 |
Application date |
AD |
May 24, 1999 |
| 21 |
Application number |
AN |
14359499 |
|
Country of application |
AC |
JP |
|
Publication date |
PUB |
Dec 21, 1999 |
33
31
32 |
Priority data |
PRC
PRN
PRD |
DE
19823655
May 27, 1998
|
33
31
32 |
PRC
PRN
PRD |
DE
19841337
Sep 10, 1998
|
33
31
32 |
PRC
PRN
PRD |
DE
29811066
Jun 23, 1998
|
| 51 |
IPC main class |
ICM |
C12N 13/00
|
| 51 |
IPC secondary class |
ICS |
C12M 1/00
C12M 1/42
C12N 15/09
|
|
IPC additional class |
ICA |
|
|
IPC index class |
ICI |
|
|
Cooperative patent classification |
CPC |
C12M 35/02
C12M 35/04
C12M 35/08
C12N 13/00
C12N 15/89
|
|
MCD main class |
MCM |
C12N 15/09
(2006.01)
|
|
MCD secondary class |
MCS |
C12M 1/00
(2006.01)
C12M 1/42
(2006.01)
C12M 3/00
(2006.01)
C12N 13/00
(2006.01)
C12N 15/89
(2006.01)
C12N 5/02
(2006.01)
|
|
MCD additional class |
MCA |
|
| 57 |
Abstract |
AB |
[EN] PROBLEM TO BE SOLVED: To easily intracellularly operate biological cells by perforating cell membranes within the range of supporting cells at spaces apart from the support edge so as to avoid positioning of the cells with costly handwork using hollow needles for the cells to be handled. SOLUTION: Biological cell(s) 3 existing in a culture medium 2 and adheredly accumulated within its support range 5 are intracellularly operated through perforating the cell(s) by such a means as electroporation, mechanical pulses or (superhigh)-ultrasonic waves with the support range 5 for the cell(s) 3 at space(s) apart from the support edge; wherein it is preferable that the cell(s) 3 are fixed within the support range 5 by the aid of suction force, and one or more kinds of substance and/or cellular component are withdrawn from and/or inserted into, via the perforation(s), the inside of the cell(s). |
| 56 |
Cited documents identified in the search |
CT |
|
| 56 |
Cited documents indicated by the applicant |
CT |
|
| 56 |
Cited non-patent literature identified in the search |
CTNP |
|
| 56 |
Cited non-patent literature indicated by the applicant |
CTNP |
|
|
Citing documents |
|
Determine documents
|
|
Sequence listings |
|
|
|
Search file IPC |
ICP |
C12M 1/26 ZEL
C12M 1/34 PC
C12M 1/42
C12N 13/00
C12N 15/89
|
