Bibliografische Daten

Dokument EP000002402433A1 (Seiten: 25)

Bibliografische Daten Dokument EP000002402433A1 (Seiten: 25)
INID Kriterium Feld Inhalt
54 Titel TI [DE] Verfahren zur Erhöhung der Expression von rekombinanten Proteinen und Ausbeute von Plasmiden durch Kultivierung von eukaryotischen oder prokaryotichen Zellen in hohen Dichten
[EN] Method for increasing the expression of a recombinant protein and plasmid yield in an eukaryotic or prokaryotic high cell density culture
[FR] Méthode d'accroissement de l'expression d'une protéine recombinante et du rendement de plasmide à haute densité dans une culture d'eucaryote ou procaryote
71/73 Anmelder/Inhaber PA BIOSILTA OY, FI
72 Erfinder IN NEUBAUER PETER, DE ; PILAREK MACIEJ, PL
22/96 Anmeldedatum AD 01.07.2010
21 Anmeldenummer AN 10168159
Anmeldeland AC EP
Veröffentlichungsdatum PUB 04.01.2012
33
31
32
Priorität PRC
PRN
PRD


51 IPC-Hauptklasse ICM C12N 1/38 (2006.01)
51 IPC-Nebenklasse ICS
IPC-Zusatzklasse ICA
IPC-Indexklasse ICI
Gemeinsame Patentklassifikation CPC C12N 1/38
MCD-Hauptklasse MCM C12N 1/38 (2006.01)
MCD-Nebenklasse MCS
MCD-Zusatzklasse MCA
57 Zusammenfassung AB [EN] The present invention relates to a method for increasing the expression of a recombinant protein and plasmid yield in an eukaryotic or prokaryotic high cell density culture. The increase of the expression is achieved by the use of perfluorochemicals as ingredients of an enzyme-based fed-batch cultivation system by enhancing the cell growth rate and the final cell density.
56 Entgegengehaltene Patentdokumente/Zitate,
in Recherche ermittelt
CT EP000000164813A2
US000003850753A
56 Entgegengehaltene Patentdokumente/Zitate,
vom Anmelder genannt
CT FI002010050648W
EP000008157594A
EP000009154440A
EP002009056861W
56 Entgegengehaltene Nichtpatentliteratur/Zitate,
in Recherche ermittelt
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GOTOH T ET AL: "Perfluorocarbon-mediated aeration applied to recombinant protein production by virus-infected insect cells", BIOCHEMICAL ENGINEERING JOURNAL, vol. 7, no. 1, 2001, pages 69 - 78, XP002597391, ISSN: 1369-703X, DOI: 10.1016/S1369-703X(00)00103-0 0;
HAMAMOTO K ET AL: "HIGH CELL DENSITY CULTURE OF A HYBRIDOMA USING PERFLUOROCARBON TO SUPPLY OXYGEN", 1 January 1987, AGRICULTURAL AND BIOLOGICAL CHEMISTRY, JAPAN SOC. FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEM, TOKYO, JP, ISSN: 0002-1369, XP009137688 0;
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LONG LIU ET AL: "Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of Streptococcus zooepidemicus", BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 32, no. 6, 11 February 2009 (2009-02-11), SPRINGER, BERLIN, DE, pages 755 - 763, XP019741069, ISSN: 1615-7605, DOI: 10.1007/S00449-009-0300-6 0;
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MATTIASSON B ET AL: "Perfluorochemicals in biotechnology", TRENDS IN BIOTECHNOLOGY, vol. 5, no. 9, 1 September 1987 (1987-09-01), ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, pages 250 - 254, XP023595464, ISSN: 0167-7799, [retrieved on 19870901], DOI: 10.1016/0167-7799(87)90101-6 0
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