Bibliographic data

Document EP000001670914B1 (Pages: 19)

Bibliographic data Document EP000001670914B1 (Pages: 19)
INID Criterion Field Contents
54 Title TI [DE] VERFAHREN ZUR SEQUENZ-SATURATIONS-MUTAGENESE (SESAM)
[EN] A PROCESS FOR SEQUENCE SATURATION MUTAGENESIS (SESAM)
[FR] PROCÉDÉ POUR LA MUTAGENÈSE DE SATURATION DE SÉQUENCE (SESAM)
71/73 Applicant/owner PA BASF AG, DE
72 Inventor IN SCHWANEBERG ULRICH, DE
22/96 Application date AD Sep 30, 2004
21 Application number AN 04787058
Country of application AC EP
Publication date PUB Jan 23, 2008
33
31
32		 Priority data PRC
PRN
PRD		 EP
2004010911
Sep 30, 2004
33
31
32		 PRC
PRN
PRD		 EP
03022311
Oct 2, 2003
51 IPC main class ICM C12N 15/10 (2006.01)
51 IPC secondary class ICS
IPC additional class ICA
IPC index class ICI
Cooperative patent classification CPC C12N 15/102
MCD main class MCM C12N 15/10 (2006.01)
MCD secondary class MCS
MCD additional class MCA
57 Abstract AB [EN] A process for the mutagenesis of a double-stranded polynucleotide sequence (master sequence) of n base-pairs having a (+)-strand and a complementary (−)-strand comprising the steps (i) creation of a collection of single-stranded fragments of the (+)-strand of the master sequence wherein all members of the collection have the same 5′-terminus and have a deletion in the 3-terminus such that the collection represents (+)-strands with a length of n−1, n−2, n−3, . . . nucleotides; (ii) introduction of at least one universal or degenerate nucleotide at the 3′-terminus of the (+) strand produced in step (i); (iii) elongation of the (+)-strand produced in step (ii) to the full length of the master sequence using the (−)-strand or fragments thereof as a template strand for the elongation; (iv) synthesis of a (−)-strand by using the (+)-strand produced in step (iii) as a template strand thereby effecting mutations in the (−)-strand at the positions of the previous universal or degenerate nucleotides compared to the master sequence.
56 Cited documents identified in the search CT
56 Cited documents indicated by the applicant CT
56 Cited non-patent literature identified in the search CTNP
56 Cited non-patent literature indicated by the applicant CTNP
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Search file IPC ICP C12P 19/34