| 54 |
Title |
TI |
[DE] Verfahren und Vorrichtung zur selektiven Lyse von zellulären Partikeln
[EN] Method for selective lysis of cellular particles in mixture, particularly blood cells by using microfluidic chip, involves subjecting particles to electromagnetic radiation that has wavelength in a specified absorption range |
| 71/73 |
Applicant/owner |
PA |
Robert Bosch GmbH, 70469, Stuttgart, DE
|
| 72 |
Inventor |
IN |
Beyl, Yvonne, 70839, Gerlingen, DE
;
Brettschneider, Thomas, 70806, Kornwestheim, DE
;
Reinhardt, Martina, 76131, Karlsruhe, DE
|
| 22/96 |
Application date |
AD |
Mar 4, 2013 |
| 21 |
Application number |
AN |
102013203655 |
|
Country of application |
AC |
DE |
|
Publication date |
PUB |
Sep 4, 2014 |
33
31
32 |
Priority data |
PRC
PRN
PRD |
|
| 51 |
IPC main class |
ICM |
G01N 15/10
(2006.01)
|
| 51 |
IPC secondary class |
ICS |
A61M 1/36
(2006.01)
G01N 1/44
(2006.01)
G01N 33/48
(2006.01)
|
|
IPC additional class |
ICA |
|
|
IPC index class |
ICI |
|
|
Cooperative patent classification |
CPC |
B01L 3/5027
C12N 1/066
C12N 13/00
G01N 1/44
|
|
MCD main class |
MCM |
G01N 15/10
(2006.01)
|
|
MCD secondary class |
MCS |
A61M 1/36
(2006.01)
G01N 1/44
(2006.01)
G01N 33/48
(2006.01)
|
|
MCD additional class |
MCA |
|
| 57 |
Abstract |
AB |
[DE] Es wird ein Verfahren und eine Vorrichtung zur Durchführung einer selektiven Lyse von zellulären Partikeln in einem Gemisch bereitgestellt. Hierbei werden die Partikel mit elektromagnetischer Strahlung (17) beaufschlagt, wobei die Wellenlänge der elektromagnetischen Strahlung (17) im Absorptionsbereich der zu lysierenden zellulären Partikel liegt.
[EN] The method involves subjecting the particles to an electromagnetic radiation (17). The wavelength of the electromagnetic radiation lies in an absorption range of the to-be-lysed cellular particles. The electromagnetic radiation is defocused. The wavelength of the electromagnetic radiation is in a range that lies between about 300 nanometer and 500 nanometer. The power density of the electromagnetic radiation is in a range that lies between about 10 megawatt per centimeter square and about 1000 megawatt per centimeter square. An independent claim is included for a device for selective lysis of the cellular particles in a mixture. |
| 56 |
Cited documents identified in the search |
CT |
US000006815209B2
US000007192560B2
US000007531138B2
US020060084165A1
WO002012136400A1
|
| 56 |
Cited documents indicated by the applicant |
CT |
|
| 56 |
Cited non-patent literature identified in the search |
CTNP |
KAUSTUBH R. RAU ET AL: "Pulsed laser microbeam-induced cell lysis: Time-resolved imaging and analysis of hydrodynamic effects", BIOPHYSICAL JOURNAL, BIOPHYSICAL SOCIETY, US, Bd. 91, Nr. 1, 1. Juli 2006 (2006-07-01), Seiten 317-329, XP002480409, ISSN: 0006-3495, DOI: 10.1529/BIOPHYSJ.105.079921 p 0
|
| 56 |
Cited non-patent literature indicated by the applicant |
CTNP |
|
|
Citing documents |
|
Determine documents
|
|
Sequence listings |
|
|
|
Search file IPC |
ICP |
A61M 1/36
G01N 1/44
G01N 15/10
G01N 33/48 H1
G01N 33/483 D2
|
