| 54 |
Title |
TI |
[DE] Verfahren zum Nachweis von HBV
[EN] Oligonucleotide primers and probes, for the detection of hepatitis B virus, are used to amplify, by polymerase chain reaction, a section of the hepatitis B virus genome |
| 71/73 |
Applicant/owner |
PA |
Drosten, Christian, 60486 Frankfurt, DE
;
Roth, W. Kurt, Priv.-Doz. Dr.med., 65185 Wiesbaden, DE
|
| 72 |
Inventor |
IN |
Drosten, Christian, 60486 Frankfurt, DE
;
Roth, W. Kurt, Priv.-Doz. Dr.med., 65185 Wiesbaden, DE
|
| 22/96 |
Application date |
AD |
Aug 7, 1998 |
| 21 |
Application number |
AN |
19835856 |
|
Country of application |
AC |
DE |
|
Publication date |
PUB |
Feb 17, 2000 |
33
31
32 |
Priority data |
PRC
PRN
PRD |
|
| 51 |
IPC main class |
ICM |
C07H 21/00
|
| 51 |
IPC secondary class |
ICS |
|
|
IPC additional class |
ICA |
|
|
IPC index class |
ICI |
|
|
Cooperative patent classification |
CPC |
C12Q 1/6823
C12Q 1/686
C12Q 1/706
|
|
MCD main class |
MCM |
|
|
MCD secondary class |
MCS |
C12Q 1/6823
(2018.01)
C12Q 1/686
(2018.01)
C12Q 1/68
(2006.01)
C12Q 1/70
(2006.01)
|
|
MCD additional class |
MCA |
|
| 57 |
Abstract |
AB |
[DE] Es wird ein Verfahren zum Nachweis von HBV in einer Probe beschrieben, welches den Schritt des Aussetzens der Probe einer Polymerase-Kettenreaktion (PCR) umfaßt, wobei das durch die PCR zu amplifizierende DNA-Stück einem bestimmten Teil des HBV-Genoms entspricht. Das Verfahren ist auf besondere Weise auf das sogenannte Taqman DOLLAR I1-System angepaßt. Hierzu werden ferner speziell angepaßte Primerpaare und Nachweissonden zur Verfügung gestellt.
[EN] A method of detecting hepatitis B virus (HBV) in a sample comprising exposing the sample to a polymerase chain reaction (PCR), where the PCR amplified DNA fragment corresponds to part of the HBV genome in the region of positions 242-482 of the HBV genome, counted from the single EcoRI cleavage site in the genome, is new. Independent claims are also included for the following: (1) oligonucleotides chosen from (I)-(IV), and complements of (III) and (IV); (2) a plasmid containing a DNA fragment having a 382 bp sequence, fully defined in the specification; and (3) a test kit to detect HBV containing an oligonucleotide composition, the necessary nucleotides for PCR and a heat stable DNA polymerase. CnAACCTSYGTCCTCCAAYTTGT (I); GATGAGGCATAGCAGCAGGAT (II); AACGCCGCAGACACATCCAGCGAT (III); and ACGACGGACCACACTGACCAGTCA (IV). n = 0 or 1; S = C or G; and Y = C or T. |
| 56 |
Cited documents identified in the search |
CT |
EP000000523557A1
EP000000569237A2
WO001990013667A1
WO001991010746A1
WO001997040193A2
|
| 56 |
Cited documents indicated by the applicant |
CT |
|
| 56 |
Cited non-patent literature identified in the search |
CTNP |
|
| 56 |
Cited non-patent literature indicated by the applicant |
CTNP |
|
|
Citing documents |
|
Determine documents
|
|
Sequence listings |
|
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Search file IPC |
ICP |
C07H 21/00
|
