| 54 |
Title |
TI |
[DE] Verfahren zur elektrochemischen Detektion von Bindungsreaktionen
[EN] Method for electrochemical detection of binding reactions
[FR] Procédé de détection électrochimique de réactions de liaison |
| 71/73 |
Applicant/owner |
PA |
FRAUNHOFER GES FORSCHUNG, DE
|
| 72 |
Inventor |
IN |
ETTLINGER JULIA, DE
;
GAJOVIC-EICHELMANN NENAD, DE
;
MICHEEL BURKHARD, DE
;
SCHARTE GUDRUN, DE
;
SCHENK JOERG, DE
|
| 22/96 |
Application date |
AD |
May 25, 2010 |
| 21 |
Application number |
AN |
10005425 |
|
Country of application |
AC |
EP |
|
Publication date |
PUB |
Nov 30, 2011 |
33
31
32 |
Priority data |
PRC
PRN
PRD |
|
| 51 |
IPC main class |
ICM |
G01N 33/543
(2006.01)
|
| 51 |
IPC secondary class |
ICS |
G01N 33/74
(2006.01)
|
|
IPC additional class |
ICA |
|
|
IPC index class |
ICI |
|
|
Cooperative patent classification |
CPC |
G01N 27/3277
G01N 33/542
G01N 33/54306
G01N 33/5438
G01N 33/743
|
|
MCD main class |
MCM |
G01N 33/542
(2006.01)
|
|
MCD secondary class |
MCS |
G01N 33/543
(2006.01)
G01N 33/74
(2006.01)
|
|
MCD additional class |
MCA |
|
| 57 |
Abstract |
AB |
[DE] Die Erfindung betrifft ein Verfahren zur Durchführung von homogenen Immunoassay-Formaten mit hochempfindlicher elektrochemischer Detektion in Lösung, dadurch gekennzeichnet, dass zwei verschiedene Konjugate als Reagenzien mit der Probe oder einem Proben-Puffer-Gemisch kombiniert werden, wobei das eine Konjugat einen Redoxmarker und ein Analytmolekül umfasst und das zweite Konjugat einen Anti-Redoxmarker-Antikörper oder ein spezifisch bindendes Fragment davon und ein den Analyten spezifisch bindendes Molekül umfasst. Das Verfahren eignet sich für wasch- und separierungsfreie Immunoassays in der Diagnostik, insbesondere für automatisierte und mikrofluidische Immunoassays. Das Verfahren eignet sich auch für preiswerte Immunoassaysysteme und Teststreifen für den Einmalgebrauch.
[EN] Carrying out homogeneous immunoassay format with electrochemical detection, in a solution, comprises combining two different conjugates as reagents with a sample or a sample-buffer mixture. The first conjugate comprises a redox marker and an analyte molecule. The second conjugate comprises an anti-redox marker antibody or its specific binding fragment, and a molecule binding specifically to the analyte. Independent claims are also included for: (1) a redox marker antibody or its fragment, which binds specifically to the redox marker, comprising ferrocene or its derivatives, osmium-bipyridine-complex or other osmium-based complexes, ruthenium-bipyridyl-complex or other ruthenium-based complexes, p-aminophenol, hexacyanoferrate (II/III), quinone or other redox markers suitable for electrochemical immunoassays, where the redox activity of the bound redox marker is largely suppressed (quenched), preferably upto greater than 90%, preferably greater than 95%, or completely; and (2) a bispecific antibody conjugate for carrying out the above method, comprising the redox marker-antibody or its fragment, and an anti-analyte-antibody or its fragment. |
| 56 |
Cited documents identified in the search |
CT |
US020040020791A1
|
| 56 |
Cited documents indicated by the applicant |
CT |
EP000000200960A1
EP000001331482A1
US000003839153A
US000004016043A
US000004960693A
US000006156271A
US020070054317A1
US020080199972A1
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| 56 |
Cited non-patent literature identified in the search |
CTNP |
FORROW NIGEL J ET AL: "Synthesis, characterization, and evaluation of ferrocene-theophylline conjugates for use in electrochemical enzyme immunoassay.", BIOCONJUGATE CHEMISTRY, vol. 15, no. 1, January 2004 (2004-01-01), pages 137 - 144, XP002593998, ISSN: 1043-1802 0;
GHINDILIS A L ET AL: "Nanomolar determination of the ferrocene derivatives using a recycling enzyme electrode: Development of a redox label immunoassay", ANALYTICAL LETTERS, vol. 28, no. 1, 1995, pages 1 - 11, XP008124810, ISSN: 0003-2719 0;
SAPIN RÉMY ET AL: "Efficacy of a new blocker against anti-ruthenium antibody interference in the Elecsys free triiodothyronine assay.", CLINICAL CHEMISTRY AND LABORATORY MEDICINE : CCLM / FESCC 2007 LNKD- PUBMED:17378744, vol. 45, no. 3, 2007, pages 416 - 418, XP008124809, ISSN: 1434-6621 0;
WEI M-Y ET AL: "Development of redox-labeled electrochemical immunoassay for polycyclic aromatic hydrocarbons with controlled surface modification and catalytic voltammetric detection", BIOSENSORS AND BIOELECTRONICS 20090515 ELSEVIER LTD; ELSEVIER ADVANCED TECHNOLOGY; THE BOULEVARD GB, vol. 24, no. 9, 15 May 2009 (2009-05-15), pages 2909 - 2914, XP002593999, DOI: 10.1016/J.BIOS.2009.02.031 0
|
| 56 |
Cited non-patent literature indicated by the applicant |
CTNP |
CHONGXIAO TAN; NENAD GAJOVIC-EICHELMANN; WALTER F.M. STÖCKLEIN; RAINER POLZIUS; FRANK F. BIER, ANALYTICA CHIMICA ACTA, vol. 658, 2010, pages 187 - 192 1;
I. COILLE; S. REDER; S. BUCHER; G. GAUGLITZ, BIOMOL. ENG, vol. 18, 2002, pages 273 - 280 1;
KATALIN DI GLERIA; H. ALLEN; 0. HILL; CALUM J. MCNEIL, ANAL. CHEM., vol. 58, 1988, pages 1203 - 1205 1;
ROGER M. NIELSON; JOSEPH T. HUPP, INORG. CHEM., vol. 35, 1996, pages 1402 - 1404 1;
SUNG JU YOO; YOUNG BONG CHOI; JONG IL JU; GUN-SIK TAE; HYUG HAN KIM; SANG-HOON LEE, ANALYST, vol. 134, 2009, pages 2462 - 2467 1
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Search file IPC |
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G01N 33/543
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