Bibliographic data

Document CN000102959397A (Pages: 15)

Bibliographic data Document CN000102959397A (Pages: 15)
INID Criterion Field Contents
54 Title TI [EN] Method for electrochemically detecting binding reactions
71/73 Applicant/owner PA FRAUNHOFER GES FORSCHUNG
72 Inventor IN BURKHARD MICHEEL ; ETTLINGER JULIA ; GAJOVIC-EICHELMANN NENAD ; SCHARTE GUDRUN ; SCHENK JOERG
22/96 Application date AD May 25, 2011
21 Application number AN 201180025601
Country of application AC CN
Publication date PUB Mar 6, 2013
33
31
32
Priority data PRC
PRN
PRD
EP
2011002586
May 25, 2011
33
31
32
PRC
PRN
PRD
EP
10005425
May 25, 2010
51 IPC main class ICM G01N 33/543 (2006.01)
51 IPC secondary class ICS G01N 33/542 (2006.01)
G01N 33/74 (2006.01)
IPC additional class ICA
IPC index class ICI
Cooperative patent classification CPC G01N 27/3277
G01N 33/542
G01N 33/54306
G01N 33/5438
G01N 33/743
MCD main class MCM G01N 33/543 (2006.01)
MCD secondary class MCS G01N 33/542 (2006.01)
G01N 33/74 (2006.01)
MCD additional class MCA
57 Abstract AB [EN] The invention relates to a method for carrying out homogenous immunoassay formats with highly sensitive electrochemical detection in solution, characterized in that two different conjugates are combined as reagents with the sample or a sample/buffer mixture, wherein one conjugate comprises a redox marker and an analyte molecule and the second conjugate comprises an anti-redox marker antibody or a specifically binding fragment thereof and a molecule that specifically binds the analyte. The method is suitable for wash- and separation-free immunoassays in diagnostics, in particular for automated and microfluidic immunoassays. The method is also suitable for inexpensive immunoassay systems and test strips that are disposable.
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