Bibliografische Daten

Dokument US000008076114B2 (Seiten: 90)

Bibliografische Daten Dokument US000008076114B2 (Seiten: 90)
INID Kriterium Feld Inhalt
54 Titel TI [EN] Cytochrome P450 oxygenases
71/73 Anmelder/Inhaber PA ARNOLD FRANCES H, US ; CALIFORNIA INST OF TECHN, US ; FARINAS EDGARDO T, US ; GLIEDER ANTON, AT ; SCHWANEBERG ULRICH, DE
72 Erfinder IN ARNOLD FRANCES H, US ; FARINAS EDGARDO T, US ; GLIEDER ANTON, AT ; SCHWANEBERG ULRICH, DE
22/96 Anmeldedatum AD 05.04.2010
21 Anmeldenummer AN 75421610
Anmeldeland AC US
Veröffentlichungsdatum PUB 13.12.2011
33
31
32
Priorität PRC
PRN
PRD
US
20121302
22.07.2002
33
31
32
PRC
PRN
PRD
US
30676601
20.07.2001
33
31
32
PRC
PRN
PRD
US
30842901
27.07.2001
33
31
32
PRC
PRN
PRD
US
80097007
07.05.2007
51 IPC-Hauptklasse ICM C12N 9/02 (2006.01)
51 IPC-Nebenklasse ICS C07H 21/04 (2006.01)
C12N 1/20 (2006.01)
C12N 15/00 (2006.01)
C12P 21/04 (2006.01)
C12Q 1/26 (2006.01)
IPC-Zusatzklasse ICA
IPC-Indexklasse ICI
Gemeinsame Patentklassifikation CPC C12N 9/0071
C12P 17/02
C12P 7/02
C12P 7/04
C12P 7/16
Y02E 50/10
Y02P 20/52
MCD-Hauptklasse MCM C12N 9/02 (2006.01)
MCD-Nebenklasse MCS C07H 21/04 (2006.01)
C12N 1/20 (2006.01)
C12N 15/00 (2006.01)
C12P 17/02 (2006.01)
C12P 21/04 (2006.01)
C12P 7/02 (2006.01)
C12P 7/04 (2006.01)
C12P 7/16 (2006.01)
C12Q 1/26 (2006.01)
MCD-Zusatzklasse MCA
57 Zusammenfassung AB [EN] Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
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