Bibliografische Daten

Dokument US000007226768B2 (Seiten: 84)

Bibliografische Daten Dokument US000007226768B2 (Seiten: 84)
INID Kriterium Feld Inhalt
54 Titel TI [EN] Cytochrome P450 oxygenases
71/73 Anmelder/Inhaber PA CALIFORNIA INST OF TECHN, US
72 Erfinder IN ARNOLD FRANCES H, US ; FARINAS EDGARDO T, US ; GLIEDER ANTON, AT ; SCHWANEBERG ULRICH, DE
22/96 Anmeldedatum AD 22.07.2002
21 Anmeldenummer AN 20121302
Anmeldeland AC US
Veröffentlichungsdatum PUB 05.06.2007
33
31
32
Priorität PRC
PRN
PRD
US
30676601
20.07.2001
33
31
32
PRC
PRN
PRD
US
30842901
27.07.2001
51 IPC-Hauptklasse ICM C12N 9/02 (2006.01)
51 IPC-Nebenklasse ICS C07H 21/04 (2006.01)
C12N 1/20 (2006.01)
C12N 15/00 (2006.01)
C12P 21/04 (2006.01)
C12Q 1/26 (2006.01)
C12Q 1/34 (2006.01)
IPC-Zusatzklasse ICA C12Q 1/00 (2006.01)
IPC-Indexklasse ICI
Gemeinsame Patentklassifikation CPC C12N 9/0071
C12P 17/02
C12P 7/02
C12P 7/04
C12P 7/16
Y02E 50/10
Y02P 20/52
MCD-Hauptklasse MCM C12N 9/02 (2006.01)
MCD-Nebenklasse MCS C07H 21/04 (2006.01)
C12N 1/20 (2006.01)
C12N 15/00 (2006.01)
C12P 17/02 (2006.01)
C12P 21/04 (2006.01)
C12P 7/02 (2006.01)
C12P 7/04 (2006.01)
C12P 7/16 (2006.01)
C12Q 1/26 (2006.01)
C12Q 1/34 (2006.01)
MCD-Zusatzklasse MCA C12Q 1/00 (2006.01)
57 Zusammenfassung AB [EN] Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
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Sequenzprotokoll
Prüfstoff-IPC ICP C12N 1/20
C12N 15/00
C12N 9/02
C12P 21/04
C12P 21/06
C12Q 1/00
C12Q 1/26
C12Q 1/34