Bibliografische Daten

Dokument EP000001670914B1 (Seiten: 19)

Bibliografische Daten Dokument EP000001670914B1 (Seiten: 19)
INID Kriterium Feld Inhalt
54 Titel TI [DE] VERFAHREN ZUR SEQUENZ-SATURATIONS-MUTAGENESE (SESAM)
[EN] A PROCESS FOR SEQUENCE SATURATION MUTAGENESIS (SESAM)
[FR] PROCÉDÉ POUR LA MUTAGENÈSE DE SATURATION DE SÉQUENCE (SESAM)
71/73 Anmelder/Inhaber PA BASF AG, DE
72 Erfinder IN SCHWANEBERG ULRICH, DE
22/96 Anmeldedatum AD 30.09.2004
21 Anmeldenummer AN 04787058
Anmeldeland AC EP
Veröffentlichungsdatum PUB 23.01.2008
33
31
32		 Priorität PRC
PRN
PRD		 EP
2004010911
30.09.2004
33
31
32		 PRC
PRN
PRD		 EP
03022311
02.10.2003
51 IPC-Hauptklasse ICM C12N 15/10 (2006.01)
51 IPC-Nebenklasse ICS
IPC-Zusatzklasse ICA
IPC-Indexklasse ICI
Gemeinsame Patentklassifikation CPC C12N 15/102
MCD-Hauptklasse MCM C12N 15/10 (2006.01)
MCD-Nebenklasse MCS
MCD-Zusatzklasse MCA
57 Zusammenfassung AB [EN] A process for the mutagenesis of a double-stranded polynucleotide sequence (master sequence) of n base-pairs having a (+)-strand and a complementary (−)-strand comprising the steps (i) creation of a collection of single-stranded fragments of the (+)-strand of the master sequence wherein all members of the collection have the same 5′-terminus and have a deletion in the 3-terminus such that the collection represents (+)-strands with a length of n−1, n−2, n−3, . . . nucleotides; (ii) introduction of at least one universal or degenerate nucleotide at the 3′-terminus of the (+) strand produced in step (i); (iii) elongation of the (+)-strand produced in step (ii) to the full length of the master sequence using the (−)-strand or fragments thereof as a template strand for the elongation; (iv) synthesis of a (−)-strand by using the (+)-strand produced in step (iii) as a template strand thereby effecting mutations in the (−)-strand at the positions of the previous universal or degenerate nucleotides compared to the master sequence.
56 Entgegengehaltene Patentdokumente/Zitate,
in Recherche ermittelt		 CT
56 Entgegengehaltene Patentdokumente/Zitate,
vom Anmelder genannt		 CT
56 Entgegengehaltene Nichtpatentliteratur/Zitate,
in Recherche ermittelt		 CTNP
56 Entgegengehaltene Nichtpatentliteratur/Zitate,
vom Anmelder genannt		 CTNP
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Prüfstoff-IPC ICP C12P 19/34